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فناوری منابع زیستی
جزئیات رویداد
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همایش ، رویداد ، ژورنال
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  • degradation of triphenylmethane dyes using a temperature and ph stable spore laccase from a novel strain of bacillus vallismortis

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    • تاریخ ارائه: 1390/01/01
    • تاریخ انتشار در تی پی بین: 1390/01/01
    • تعداد بازدید: 669
    • تعداد پرسش و پاسخ ها: 0
    • شماره تماس دبیرخانه رویداد: -
     the characterization of a spore laccase from bacillus vallismortis fmb-103, isolated from textile industry disposal sites, is described. the activity was 6.5 u/g of dry spore with abts as the substrate. the enzyme was quite stable at high temperature. it retained more than 90% of its initial activity after 10 h at 70 °c. the enzyme demonstrated broad ph stability in both acidic and alkaline conditions. there was almost no activity loss at ph 3 over an extended period of time, and the relative activity remained at 82% and 38% at ph 7 and ph 9 after 10 days. nan3, sds, l-cysterine, dithiothreitol, edta and nacl inhibit the enzyme activity. triphenylmethane dyes, including malachite green, brilliant green and aniline blue were efficiently degraded by the enzyme after 24 h in combination with a mediator with efficiencies of 76.84%, 96.56% and 81.17%, respectively. the reusability of spore laccase for decolorization dyes was also examined.

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