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جزئیات رویداد
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همایش ، رویداد ، ژورنال
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  • effective immobilisation of lipase to enhance esterification potential and reusability

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    • تاریخ ارائه: 1392/07/24
    • تاریخ انتشار در تی پی بین: 1392/07/24
    • تعداد بازدید: 976
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    • شماره تماس دبیرخانه رویداد: -
     a commercial lipase, “lipolase t100”, was immobilised onto silica by means of physical adsorption. the silica-bound lipase was subsequently exposed to 1 vol. % glutaraldehyde (pentane-1,5-dial). the silica was loaded repeatedly with the lipolase t100 in 0.05 m tris buffer (ph 8.5) until saturation was achieved. during the 1st, 2nd, 3rd, 4th, and 5th cycles of loading of silica with the enzyme, the protein-binding on the silica achieved 51.73 %, 48.27 %, 26.92 %, 10.73 %, and 4.29 %, respectively. the synthesis of methyl salicylate (methyl 2-hydroxybenzoate) and linalyl ferulate (3,7-dimethylocta-1,6-dien-3-yl 4-hydroxy-3-methoxycinnamate) carried out at 45°c under shaking with mole ratios of 200 mm of acid and 500 mm alcohol in dmso using 15 mg ml−1 of hyper-activated biocatalyst resulted in yield(s) of 77.2 % of methyl salicylate and 65.3 % of linalyl ferulate in the presence of molecular sieves. the hyper-activated biocatalyst was more efficient than the previously reported silica-bound lipase with minimum leaching of the enzyme from the reaction mixture. the km and v max of the free (0.142 mm and 38.31 μmol min−1 ml−1, respectively) and silica-bound lipase (0.043 mm and 26.32 μmol min−1 mg−1, respectively) were determined for the hydrolysis of p-npp. during repeated esterification studies using silica-bound lipase, yields of 50.1 % of methyl salicylate after the 5th cycle, and 53.9 % of linalyl ferulate after the 7th cycle of esterification were recorded. in the presence of molecular sieves (30 mg ml−1) in the reaction mixture, the maximum syntheses of methyl salicylate (77.2 %) and linalyl ferulate (65.3 %) were also observed. in a volumetric batch scale-up, when the reaction volume was increased to 50 ml, 44.9 % and 31.4 % yields of methyl salicylate and linalyl ferulate, respectively, were achieved.

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