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جزئیات رویداد
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  • effect of salicin on induction and carbon catabolite repression of endoxylanase synthesis in penicillium janthinellum mtcc 10889

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    • تاریخ ارائه: 1392/07/24
    • تاریخ انتشار در تی پی بین: 1392/07/24
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     amongst various carbon sources, xylan was found to be the sole inducer of endoxylanase production by penicillium janthinellum mtcc 10889 in submerged cultivation. endoxylanase synthesis by a xylan induced culture was initially repressed after a simultaneous addition of xylose, probably by the inducer exclusion mechanism, but it was resumed and achieved its highest level at a much later stage of growth (at 120 h). xylose added after 30 h of growth cannot exert its full repressive effect. although glucose was proved to be a more potent repressor than xylose, supplementation of salicin, an alcoholic β-glycoside containing d-glucose, with pure xylan resulted in an about 3.22 fold increase in the enzyme synthesis at 72 h followed by constant high production of the enzyme at least until the 144th h of growth. inducing capacity of salicin in a xylan induced culture was significantly reduced when it was added after 30 h of growth. addition of salicin and xylan help to partially overcome the repressive effect of xylose and glucose. failure of salicin in recovering the endoxylanase synthesis in actinomycin d and cyclohexamide inhibited the xylan induced culture indicating that salicin cannot initiate the de novo synthesis of the enzyme.

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