• interaction of oligonucleotides with benzo[c]phenanthridine alkaloid sanguilutine

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    جزئیات بیشتر مقاله
    • تاریخ ارائه: 1392/07/24
    • تاریخ انتشار در تی پی بین: 1392/07/24
    • تعداد بازدید: 782
    • تعداد پرسش و پاسخ ها: 0
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     benzo[c]phenanthridine alkaloid sanguilutine was extracted and purified from the dried roots ofsanguinaria canadensis. the interaction of the positively charged iminium form of alkaloid with double-stranded dna oligonucleotides was studied using luminescence spectroscopy. the results showed that the interaction with various double-stranded oligonucleotides was not specific to a-t or g-c base pairs; also, no preference was found for either homogeneous or heterogeneous base composition of strands. the association constants were calculated to be in the range of (1.31–14.36) × 105 m−1. the luminescence intensity response at 610 nm to low concentrations of double-stranded dna was found to be linear and can potentially be used for the fluorometric quantification of dna. the limit of detection was estimated to be 120 ng ml−1 of dna (calculated by 3σ method).

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