• (human umbilical cord mesenchymal stem cells inhibit c6 glioma growth via secretion of dickkopf-1 (dkk1

    جزئیات بیشتر مقاله
    • تاریخ ارائه: 1392/07/24
    • تاریخ انتشار در تی پی بین: 1392/07/24
    • تعداد بازدید: 803
    • تعداد پرسش و پاسخ ها: 0
    • شماره تماس دبیرخانه رویداد: -
    mesenchymal stem cells (mscs) represent a potential therapeutic target for glioma. we determined the molecular mechanism of inhibitory effect of human umbilical cord-derived mscs (huc-mscs) on the growth of c6 glioma cells. we demonstrated that huc-mscs inhibited c6 cell growth and modulated the cell cycle to g0/g1 phase. the expression of β-catenin and c-myc was downregulated in c6 cells by conditioned media from huc-mscs, and the levels of secreted dkk1 were positively correlated with concentrations of hucmscs-cm. the inhibitory effect of huc-mscs on c6 cell proliferation was enhanced as the concentration of dkk1 in hucmscs-cm increased. when dkk1 was neutralized by anti-dkk1 antibody, the inhibitory effect of huc-mscs on c6 cells was attenuated. furthermore, we found that conditioned media from huc-mscs transfection with sirna targeting dkk1 mrna or pegfpn1-dkk1 plasmid lost or enhanced the abilities to regulate the wnt signaling in c6 cells. therefore, huc-mscs inhibited c6 glioma cell growth via secreting dkk1, an inhibitor of wnt pathway, may represent a novel therapeutic strategy for malignant glioma.

سوال خود را در مورد این مقاله مطرح نمایید :

با انتخاب دکمه ثبت پرسش، موافقت خود را با قوانین انتشار محتوا در وبسایت تی پی بین اعلام می کنم