مقاله Modification of cardiac RYR2 gating by a peptide from the central domain of the RYR2 : تی پی بین

خلاصه مقاله

The effect of a domain peptide DPCPVTc from the central region of the RYR2 on ryanodine receptors from rat heart has been examined in planar lipid bilayers. At a zero holding potential and at 8 mmol L−1 luminal Ca2+ concentration, DPCPVTc induced concentrationdependent activation of the ryanodine receptor that led up to 20-fold increase of PO at saturating DPCPVTc concentrations. DPCPVTcprolonged RyR2 openings and increased RyR2 opening frequency. At all peptide concentrations the channels displayed large variability in open probability, open time and frequency of openings. With increasing peptide concentration, the fraction of high open probability records increased together with their open time. The closed times of neither low- nor high-open probability records depended on peptide concentration. The concentration dependence of all gating parameters had EC50 of 20 μmol L−1 and a Hill slope of 2. Comparison of the effects of DPCPVTc with the effects of ATP and cytosolic Ca2+ suggests that activation does not involve luminal feed-through and is not caused by modulation of the cytosolic activation A-site. The data suggest that although “domain unzipping” by DPCPVTcoccurs in both modes of RyR activity, it affects RyR gating only when the channel resides in the H-mode of activity.

نویسندگان

Andrea Faltinová [ Department of Muscle Cell Research, Institute of Molecular Physiology and Genetics, Slovak Academy of Sciences, 833 34, Bratislava, Slovakia ]

Alexandra Zahradníková [ Department of Muscle Cell Research, Institute of Molecular Physiology and Genetics, Slovak Academy of Sciences, 833 34, Bratislava, Slovakia ]

کلمات کلیدی

Ryanodine receptor Planar lipid bilayer Domain peptide Allosteric activation

آدرس این مقاله

آدرس مستقیم دسترسی به این مقاله

مشخصات کنفرانس

مشاهده مقالات

Central European Journal of Biology 2014

Central European Journal of Biology (CEJB) is an English-language journal devoted to rapid publication of original research and review articles in all biology fields. The aim of this journal is to become the premier source of high quality research from Central and Eastern Europe; however, contributions are welcome from all over the world. The journal is published bimonthly. Central European Journal of Biology presents rigorously peer-reviewed scientific articles in the following categories: Research Articles, Communications, Reviews and Mini-Reviews, Commentaries and Letters to the Editor. Coverage includes Cellular and Molecular Life Sciences, Biomedical Sciences, Plant Sciences, Animal Sciences and Evolution, Ecology Environment. The aim of the editors is to publish timely, useful and informative results that will be of value to a general biological audience. We provide free language assistance to the authors of all accepted articles. Every article published in our journal is promoted to researchers working in the respective field.

حوزه های تحت پوشش : Life Sciences, general, Cell Biology, Biochemistry, general, Human Genetics, Immunology