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مجله زیست شناسی اروپای مرکزی 2014
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  • effects of nitrogen on growth and carbohydrate formation in porphyridium cruentum

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    • تاریخ ارائه: 1392/07/24
    • تاریخ انتشار در تی پی بین: 1392/07/24
    • تعداد بازدید: 747
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     the microalga porphyridium cruentum (rhodophyta) has several industrial and pharmaceutical uses, especially for its polysaccharide production. this study aimed to investigate the influence of nitrogen levels as reflected by altered n:p ratios on the production and content of biomass and carbohydrate. n:p molar ratios were altered in batch cultures to range from 1.6 to 50 using the redfield ratio of 1:16 as reference. algal growth (estimated as final cell number, biomass concentration and maximum specific growth rate) was negatively affected at low n:p ratios. the optimal n:p ratio for growth was identified at 35–50, with specific growth rates of 0.19 day−1 and maximum cell concentrations of 59·108 cells l−1 and 1.2 g dry weight of biomass l−1. in addition, variation in cell size was seen. cells with larger diameters were at higher n:p ratios and smaller cells at lower ratios. the cellular carbohydrate content increased under reduced nitrogen availability. however, because accumulation was moderate at the lowest n:p ratio, 0.4 g per g dry weight biomass compared to 0.24 at the redfield ratio of 16:1, conditions for increased total carbohydrate formation were identified at the n:p ratios optimal for growth. additionally, carbohydrates were largely accumulated in late exponential to stationary phase.

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