مقاله Characterization of natural, decellularized and reseeded porcine tooth bud matrices : تی پی بین

خلاصه مقاله

Dental tissue engineering efforts have yet to identify scaffolds that instruct the formation of bioengineered teeth of predetermined size and shape. Here we investigated whether extracellular matrix (ECM) molecules present in natural tooth scaffolds can provide insight on how to achieve this goal. We describe methods to effectively decellularize and demineralize porcine molar tooth buds, while preserving natural ECM protein gradients. Natural tooth ECM composition was assessed using histological and immunohistochemical (IHC) analyses of fibrillar and basement membrane proteins. Our results showed that Collagen I, Fibronectin, Collagen IV, and Laminin gradients were detected in natural tooth tissues, and retained in decellularized samples. Second harmonic generation (SHG) image analysis and 3D reconstructions were used to show that natural tooth tissue exhibited higher collagen fiber density, and less oriented and less organized collagen fibers, as compared to decellularized tooth tissue. We also found that reseeded decellularized tooth scaffolds exhibited distinctive collagen content and organization as compared to decelluarized scaffolds. Our results show that SHG allows for quantitative assessment of ECM features that are not easily characterized using traditional histological analyses. In summary, our results demonstrate the potential for natural decellularized molar tooth ECM to instruct dental cell matrix synthesis, and lay the foundation for future use of biomimetic scaffolds for dental tissue engineering applications.

نویسندگان

Samantha B. Traphagen [ Department of Oral and Maxillofacial Pathology, Division of Craniofacial and Molecular Genetics, Tufts University, Boston, MA 02111, USA ]

Nikos Fourligas [ Biomedical Engineering Department, Tufts University, 4 Colby Street, Medford, MA 02155, USA ]

Joanna F. Xylas [ Biomedical Engineering Department, Tufts University, 4 Colby Street, Medford, MA 02155, USA ]

Sejuti Sengupta [ Department of Oral and Maxillofacial Pathology, Division of Craniofacial and Molecular Genetics, Tufts University, Boston, MA 02111, USA ]

David L. Kaplan [ Biomedical Engineering Department, Tufts University, 4 Colby Street, Medford, MA 02155, USA ]

کلمات کلیدی

Decellularization Second harmonic generation (SHG) Tooth bud Extracellular matrix

آدرس این مقاله

آدرس مستقیم دسترسی به این مقاله

مشخصات کنفرانس

مشاهده مقالات

Biomaterials

Biomaterials is an international journal covering the science and clinical application of biomaterials. A biomaterial is now defined as a substance that has been engineered to take a form which, alone or as part of a complex system, is used to direct, by control of interactions with components of living systems, the course of any therapeutic or diagnostic procedure. It is the aim of the journal to provide a peer-reviewed forum for the publication of original papers and authoritative review and opinion papers dealing with the most important issues facing the use of biomaterials in clinical practice.

حوزه های تحت پوشش : biomaterials