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  • long-term maintenance of mouse embryonic stem cell pluripotency by manipulating integrin signaling within 3d scaffolds without active stat3

    جزئیات بیشتر مقاله
    • تاریخ ارائه: 1391/01/01
    • تاریخ انتشار در تی پی بین: 1391/01/01
    • تعداد بازدید: 620
    • تعداد پرسش و پاسخ ها: 0
    • شماره تماس دبیرخانه رویداد: -
     we engineered an acellular biomimetic microenvironment to regulate stem cell fate and applied it to maintain mouse embryonic stem (es) cell self-renewal. in the 3d environment formed using hydrogel scaffolds in which specific integrin ligation was provided, stat3 activation by exogenous leukemia inhibitory factor (lif) no longer acted as a limiting factor for stem cell self-renewal. instead, simultaneous stimulation of integrins α5β1, αvβ5, α6β1 and α9β1 within the 3d scaffold greatly increased akt1 and smad 1/5/8 activation, which resulted in prolonged self-renewal of the es cells. the es cells exposed to the combined stimulation of the integrins for 4 wk in lif-free 3d scaffolds maintained the spherical morphology of cell colonies without losing any activity of pluripotency. in conclusion, cell niche-specific integrin signaling within the 3d environment supported mouse es cell self-renewal, and the resulting integrin signaling replaced stat3 with akt1 and smad 1/5/8 as critical signals for mouse es cell self-renewal.

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