محبوبترین نویسندگان :
نویسندگان مقالات پربازدید :
مواد زیستی
جزئیات رویداد
اینستاگرام تی پی بین
همایش ، رویداد ، ژورنال
حوزه های تحت پوشش رویداد

  • directed endothelial cell morphogenesis in micropatterned gelatin methacrylate hydrogels

    نویسندگان :
    کلمات کلیدی :
    جزئیات بیشتر مقاله
    • تاریخ ارائه: 1391/01/01
    • تاریخ انتشار در تی پی بین: 1391/01/01
    • تعداد بازدید: 768
    • تعداد پرسش و پاسخ ها: 0
    • شماره تماس دبیرخانه رویداد: -
     engineering of organized vasculature is a crucial step in the development of functional and clinically relevant tissue constructs. a number of previous techniques have been proposed to spatially regulate the distribution of angiogenic biomolecules and vascular cells within biomaterial matrices to promote vascularization. most of these approaches have been limited to two-dimensional (2d) micropatterned features or have resulted in formation of random vasculature within three-dimensional (3d) microenvironments. in this study, we investigate 3d endothelial cord formation within micropatterned gelatin methacrylate (gelma) hydrogels with varying geometrical features (50–150 μm height). we demonstrated the significant dependence of endothelial cells proliferation, alignment and cord formation on geometrical dimensions of the patterned features. the cells were able to align and organize within the micropatterned constructs and assemble to form cord structures with organized actin fibers and circular/elliptical cross-sections. the inner layer of the cord structure was filled with gel showing that the micropatterned hydrogel constructs guided the assembly of endothelial cells into cord structures. notably, the endothelial cords were retained within the hydrogel microconstructs for all geometries after two weeks of culture; however, only the 100 μm-high constructs provided the optimal microenvironment for the formation of circular and stable cord structures. our findings suggest that endothelial cord formation is a preceding step to tubulogenesis and the proposed system can be used to develop organized vasculature for engineered tissue constructs.

سوال خود را در مورد این مقاله مطرح نمایید :

با انتخاب دکمه ثبت پرسش، موافقت خود را با قوانین انتشار محتوا در وبسایت تی پی بین اعلام می کنم
مقالات جدیدترین رویدادها
مقالات جدیدترین ژورنال ها