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  • reconstitution of laminin-111 biological activity using multiple peptide coupled to chitosan scaffolds

    جزئیات بیشتر مقاله
    • تاریخ ارائه: 1391/01/01
    • تاریخ انتشار در تی پی بین: 1391/01/01
    • تعداد بازدید: 823
    • تعداد پرسش و پاسخ ها: 0
    • شماره تماس دبیرخانه رویداد: -
     laminin-111, a multifunctional matrix protein, has diverse biological functions. previously, we have identified various biologically active sequences in laminin-111 by a systematic peptide screening. we also demonstrated that peptide-conjugated chitosan matrices enhance the biological functions of the active sequences and are useful as a scaffold. here, we conjugated sixty biologically active laminin-111 peptides onto chitosan matrices. the twenty-nine peptide-chitosan matrices promoted various biological activities, including cell attachment, spreading, and neurite outgrowth. the biological activities of peptide-chitosan matrices depend on the peptide. these peptide-chitosan matrices are categorized into six groups depending on their biological activities. next, we conjugated five active peptides, which showed strong cell attachment activity in the each group, onto a single chitosan matrix to mimic the multiple activities of laminin-111. the mixed peptides-chitosan matrix significantly promoted cell attachment and cell spreading over that observed with the individual peptides. we also demonstrated that a mixed peptides-chitosan matrix, using four neurite outgrowth-promoting peptides each from a different group, enhanced the activity. these data suggest that the mixed peptides synergistically induce laminin-like biological activities on a chitosan matrix. the active peptides-chitosan matrices described here have potential for use as biomaterial for tissue engineering and regeneration.

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