محبوبترین نویسندگان :
نویسندگان مقالات پربازدید :
آکتا بیومتریال
جزئیات رویداد
اینستاگرام تی پی بین
همایش ، رویداد ، ژورنال
حوزه های تحت پوشش رویداد

  • orientation of human osteoblasts on hydroxyapatite-based microchannels

    نویسندگان :
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    جزئیات بیشتر مقاله
    • تاریخ ارائه: 1392/01/01
    • تاریخ انتشار در تی پی بین: 1392/01/01
    • تعداد بازدید: 869
    • تعداد پرسش و پاسخ ها: 0
    • شماره تماس دبیرخانه رویداد: -
     the effect of calcium phosphate-based microchannels on the growth and orientation of human osteoblast cells is investigated in this study. as substrates, hydroxyapatite-based microchannels with high contouring accuracy were fabricated by a novel micro-moulding technique. microchannels obtained by this method featured widths ranging from 16.0 ± 0.7 to 76.6 ± 1.4 μm and depths from 7.9 ± 0.8 to 15.5 ± 1.3 μm. surface and contour characterization was carried out using x-ray diffraction analysis, scanning electron microscopy imaging and 3d-confocal profilometry. cell activity and alignment on microchannels with different widths were determined after 1 and 3 days by photometric spectroscopy and fluorescence microscopic imaging and statistically analysed by tukey’s multiple comparison test. on days 1 and 3 for microchannels of width 16 and 30 μm, 70–80% of the osteoblasts oriented within an angular range of 0–15° relative to the microchannel direction. interestingly, only 20% of the cells grew inside the microchannels for channel widths of 16 and 30 μm. substrates with channel widths of 45, 65 and 76 μm allowed ∼40% of the cells to grow inside. the depth of the microchannel showed hardly any significant impact. all micropatterned surfaces provoked a good cell attachment, as flat and spread cell morphologies with lamellipodiae and filopodiae could already be observed after 1 day. the effect of the microchannels on osteoblast activity was determined using the colorimetric wst-1 assay. in addition, the cell differentiation was assessed by collagen type i staining. the cell activity obtained by wst-1 assay differed insignificantly for all micropatterned samples of various widths and depths. the assessment of collagen type i yielded the same amounts for all micropatterned samples after 1, 3 and 7 days. in summary, the microchannel width of ha-based patterns has a distinct effect on the directed growth of human osteoblast cells, allowing novel design strategies for surfaces such as dental implants.

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