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آکتا بیومتریال
جزئیات رویداد
همایش ، رویداد ، ژورنال
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  • nanopatterned polymer substrates promote endothelial proliferation by initiation of β-catenin transcriptional signaling

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    • تاریخ ارائه: 1392/01/01
    • تاریخ انتشار در تی پی بین: 1392/01/01
    • تعداد بازدید: 704
    • تعداد پرسش و پاسخ ها: 0
    • شماره تماس دبیرخانه رویداد: -
     control of endothelial phenotype involves a variety of signaling pathways and transcriptional regulators, including the junctional protein β-catenin. this multifunctional signaling molecule is part of adhesion contacts in the endothelium and is able to translocate into the nucleus to activate genetic programs and control proliferation and the fate of the cells. we investigated the influence of laser-generated nanopatterns on polymeric cell culture substrates on endothelial tissue architecture, proliferation and β-catenin signaling. for our experiments human microvascular endothelial cells or cd34+endothelial progenitor cells, isolated from human adipose tissue, were cultured on polyethylene terephthalate (pet) substrates with oriented nanostructures with lateral periodicities of 1.5 μm and 300 nm, respectively. the surface topography and chemistry of the pet substrates were characterized by electron microscopy, atomic force microscopy, water contact angle measurement and x-ray photoelectron spectroscopy. analysis of cell phenotype markers as well as β-catenin signaling revealed that short-term culture of endothelial cells on nanostructured substrates generates a proliferative cell phenotype associated with nuclear accumulation of β-catenin and activation of specific β-catenin target genes. the effects of the nanostructures were not directly correlated with nanostructure-induced alignment of cells and were also clearly distinguishable from the effects of altered pet surface chemistry due to photomodification. in summary, we present a novel mechanism of surface topology-dependent control of transcriptional programs in mature endothelium and endothelial progenitor cells.

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