• an in situ cross-linking hybrid hydrogel for controlled release of proteins

    جزئیات بیشتر مقاله
    • تاریخ ارائه: 1392/01/01
    • تاریخ انتشار در تی پی بین: 1392/01/01
    • تعداد بازدید: 674
    • تعداد پرسش و پاسخ ها: 0
    • شماره تماس دبیرخانه رویداد: -
     there is a clear need for methods to provide a safe controlled release of therapeutic proteins, either to achieve and maintain high local protein concentrations, or for sustained systemic delivery. we have developed a protein delivery system that combines in situ cross-linkable polysaccharide hydrogels with gelatin. this formulation is injectable, easy to apply, and obviates the need for organic solvents or potentially toxic cross-linking agents in the formulation process. the cross-linked polysaccharides themselves (comprising hyaluronic acid, dextran and/or carboxymethylcellulose) provided prolonged release of fluorescently labeled albumin (fitc-albumin). the duration of release was markedly extended by the incorporation of gelatin into the formulation: fitc-albumin and interleukin-2 (il-2) were released over the course of more than 3 weeks. the il-2 maintained >70% activity throughout that time. gelatin also accelerated the gelation time of the hydrogels, and reduced their swelling in phosphate-buffered saline. the composite hydrogel (dextran–carboxymethylcellulose–gelatin) showed minimal cytotoxicity in vitro, and benign tissue reaction after subcutaneous injection in rats.

سوال خود را در مورد این مقاله مطرح نمایید :

با انتخاب دکمه ثبت پرسش، موافقت خود را با قوانین انتشار محتوا در وبسایت تی پی بین اعلام می کنم