مقاله Interplay of cell adhesion matrix stiffness and cell type for non-viral gene delivery : تی پی بین

خلاصه مقاله

Non-viral gene delivery has the potential to treat a wide array of diseases but has been hindered by limited expression in vivo, possibly due to complex cellular microenvironments at delivery sites. Previous studies have reported that extracellular matrix properties, including stiffness, influence non-viral gene transfection efficiencies. This study reports that the effect of matrix stiffness on non-viral gene delivery differs among cell types due to varying sensitivities to matrix rigidity. Plasmid DNA encoding bone morphogenetic protein (BMP)-2 was delivered to fibroblasts, bone marrow stromal cells, and myoblasts cultured on fibronectin-conjugated poly(ethylene glycol) diacrylate hydrogels with varied elastic moduli, and the cellular uptake and subsequent expression of plasmid DNA were examined. While exogenous BMP-2 expression increased with increasing matrix stiffness for all three cell types, the effects of matrix stiffness were most pronounced for fibroblasts. Mechanistic studies conducted in parallel indicate that matrix stiffness influenced the projected area and nuclear aspect ratio for fibroblasts but had minimal effects on the morphology of bone marrow stromal cells and myoblasts. Overall, we believe that the results of this study will be useful for developing advanced non-viral gene delivery strategies for improved therapeutic efficacy.

نویسندگان

Cathy Chu [ Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, 114 Roger Adams Laboratory, 600 South Mathews Avenue, Urbana, IL 61801, USA ]

Hyunjoon Kong [ Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, 114 Roger Adams Laboratory, 600 South Mathews Avenue, Urbana, IL 61801, USA ]

کلمات کلیدی

(Gene therapy ECM (extracellular matrix) Hydrogel Mechanical properties BMP (bone morphogenetic protein

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Acta Biomaterialia

Acta Biomaterialia is an international journal that publishes peer-reviewed original research reports, review papers and communications in the broadly defined field of biomaterials science. The emphasis of the journal is on the relationship between biomaterial structure and function at all length scales. The journal is committed to rapid peer-review and publication.

حوزه های تحت پوشش : biomaterials science