• culturing ins-1 cells on cdpgyigsr-, rgd- and fibronectin surfaces improves insulin secretion and cell proliferation

    جزئیات بیشتر مقاله
    • تاریخ ارائه: 1392/01/01
    • تاریخ انتشار در تی پی بین: 1392/01/01
    • تعداد بازدید: 703
    • تعداد پرسش و پاسخ ها: 0
    • شماره تماس دبیرخانه رویداد: -
     rat insulinoma cells (ins-1), an immortalized pancreatic beta cell line, were cultured on low-fouling carboxymethyl-dextran (cmd) layers bearing fibronectin, the tripeptide arg–gly–asp (rgd) or cdpgyigsr, a laminin nonapeptide. ins-1 cells were non-adherent on cmd and rge but adhered to fibronectin- and peptide-coated cmd surfaces and to tissue culture polystyrene (tcps). on cmd bearing fibronectin and the peptides, ins-1 cells showed higher glucose-stimulated insulin secretion compared to those on tcps, bare cmd and rge. ins-1 cells experienced a net cell growth, with the lowest found after 7 days on cmd and the highest on fibronectin. similarly, cells on rgd and cdpgyigsr showed lower net growth rates than those on fibronectin. expression of e-cadherin and integrins αvβ3 and α5 were similar between the conditions, except for α5expression on fibronectin, rgd and cdpgyigsr. larger numbers of ki-67-positive cells were found on cdpgyigsr, tcps, fibronectin and rgd. cells in all conditions expressed pdx1.

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