role of gallium and silver from phosphate-based glasses on in vitro dual species oral biofilm models of porphyromonas gingivalis and streptococcus gordonii

phosphate-based glasses (pbgs) are excellent controlled delivery agents for antibacterial ions such as silver and gallium. the aim of this study was to assess the potential utility of novel pbgs combining both gallium and silver for use in periodontal therapy. to this end, an in vitro biofilm model with the putative periodontal pathogen,porphyromonas gingivalis, and an initial colonizer, streptococcus gordonii, was established. the effect of increasing calcium content in gallium–silver-doped pbg on the susceptibility of p. gingivalis was examined. a decrease in degradation rates (30.34, 25.19, 21.40 μg mm⁻² h⁻¹) with increasing pbg calciumcontent (10, 11, 12 mol.% respectively) was observed, correlating well with gallium and silver ion release and antimicrobial activity against planktonic p. gingivalis (approximately 5.4 log10 colony-forming units (cfu) reduction after 24 h by the c10 glass compared with controls) and s. gordonii (total growth inhibition after 32 h by c10, c11 and c12 glasses compared with controls). the most potent pbg (c10) was evaluated for its ability to inhibit the biofilm growth of p. gingivalis in a newly established constant-depth film fermentor model. the simultaneous release of silver and gallium from the glass reduced p. gingivalis biofilm growth with a maximum effect (1.92 log₁₀ cfu reduction) after 168 h. given the emergence of antibiotic-resistant bacteria and dearth of new antibiotics in development, the glasses, especially c10, would offer effective alternatives to antibiotics or may complement current therapies through controlled, localized delivery of gallium and silver ions at infected sites in the oral cavity.