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  • separation of polymeric galactoglucomannans from hot-water extract of spruce wood

    جزئیات بیشتر مقاله
    • تاریخ ارائه: 1390/01/01
    • تاریخ انتشار در تی پی بین: 1390/01/01
    • تعداد بازدید: 538
    • تعداد پرسش و پاسخ ها: 0
    • شماره تماس دبیرخانه رویداد: -
     two methods for separation of polymeric galactoglucomannans (ggms) from a hot-water extract of spruce wood, i.e., membrane filtration and precipitation in ethanol–water, were compared. filtration through a series of membranes with different pore sizes separated ggms of different molar masses, from polymers to oligomers. only polysaccharides were precipitated in ethanol–water. with the optimal water content of 5–15%, the precipitated amount was about 6% on wood basis. the average molar mass of the precipitated polysaccharides was 10–12 kda with a molar mass range of 4–20 kda. ggms comprised about 80% of the precipitated hemicelluloses. other precipitated polysaccharides were mainly arabinoglucuronoxylans and pectins (rhamnogalacturonans). analysis of a lignin-free, ethanol-precipitated ggm preparation by 13c nmr spectroscopy verified that it was structurally almost identical with a ggm-rich ethanol precipitate obtained from spruce wood by extraction at much milder conditions, 90 °c for 60 min.

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