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  • combined utilization of lipase-displaying pichia pastoris whole-cell biocatalysts to improve biodiesel production in co-solvent media

    جزئیات بیشتر مقاله
    • تاریخ ارائه: 1390/01/01
    • تاریخ انتشار در تی پی بین: 1390/01/01
    • تعداد بازدید: 868
    • تعداد پرسش و پاسخ ها: 0
    • شماره تماس دبیرخانه رویداد: -
     lipase-displaying whole cells appear to be efficient biocatalysts because of their low preparation costs and simple recycling procedure. the combined utilization of candida antarctica lipase b (calb) and rhizomucor miehei lipase (rml), separately displayed on pichia pastoris whole cells, to produce biodiesel in co-solvent media was investigated. a response surface methodology incorporating a d-optimal design was employed to obtain the optimum reaction conditions for methyl ester (me) synthesis. the synergistic effect of the two displayed lipases and the use of tert-butanol and isooctane as the co-solvent media were found to significantly improve the transesterification reaction. scaled-up reactions using various types of feedstock were carried out in a 0.5–l stirred reactor under optimum conditions, affording me yields over 90% in 12 h. moreover, the me yields remained above 85% after 20 repeated batch cycles. in conclusion, this biocatalyst affords a promising route to efficient biodiesel production.

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