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  • preparation and characterization of porous cross linked laccase aggregates for the decolorization of triphenyl methane and reactive dyes

    جزئیات بیشتر مقاله
    • تاریخ ارائه: 1390/01/01
    • تاریخ انتشار در تی پی بین: 1390/01/01
    • تعداد بازدید: 591
    • تعداد پرسش و پاسخ ها: 0
    • شماره تماس دبیرخانه رویداد: -
     the production of porous cross-linked enzyme aggregates (p-cleas) is a simple and effective methodology for laccase immobilization. a three-phase partitioning technique was applied to co-precipitate laccase and starch, followed by cross-linking with glutaraldehyde and removal of starch by α-amylase to create pores in the cleas. scanning electron microscopy revealed a very smooth spherical structure with numerous large pores. the half-life of free laccase at 55 °c was calculated to be 1.3 h, while p-cleas did not lose any activity even after 14 h. p-cleas also exhibited improved storage stability, catalytic efficiency and could be recycled 15 times with 60% loss of activity. the catalysts decolorized triphenylmethane and reactive dyes by 60–70% at initial dye concentrations of 2 and 0.5 g l−1, respectively, without any mediators. these results suggest the potential of clea technology in waste-water treatment.

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