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  • fungal pretreatment of cornstalk with phanerochaete chrysosporium for enhancing enzymatic saccharification and hydrogen production

    جزئیات بیشتر مقاله
    • تاریخ ارائه: 1390/01/01
    • تاریخ انتشار در تی پی بین: 1390/01/01
    • تعداد بازدید: 538
    • تعداد پرسش و پاسخ ها: 0
    • شماره تماس دبیرخانه رویداد: -
     the feasibility of fungal pretreatment of cornstalk with phanerochaete chrysosporium for enzymatic saccharification and h2 production was investigated in this study. firstly, cornstalk was pretreated with p. chrysosporium at 29 °c under static condition for 15 d, lignin reduction was up to 34.3% with holocellulose loss less than 10%. microscopic structure observation combined ftir analysis further demonstrated that the lignin and crystallinity were decreased. subsequently, the fungal-pretreated cornstalk was subjected to enzymatic hydrolysis by the crude cellulase from trichoderma viride to produce fermentable sugars which were then fermented to bio-h2 usingthermoanaerobacterium thermosaccharolyticum w16. the maximum enzymatic saccharification was found to be 47.3% which was 20.3% higher than the control without pretreatment. upon fermentation of enzymatic hydrolysate, the yield of h2 was calculated to be 80.3 ml/g-pretreated cornstalk. the present results suggested the potential of using hydrogen-producing bacteria for high-yield conversion of cornstalk into bio-h2 integrate with biological pretreatment and enzymatic saccharification.

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