chemometric formulation of bacterial consortium-avs for improved decolorization of resonance-stabilized and heteropolyaromatic dyes

a bacterial consortium-avs, consisting of pseudomonas desmolyticum ncim 2112, kocuria rosea mtcc 1532 and micrococcus glutamicus ncim 2168 was formulated chemometrically, using the mixture design matrix based on the design of experiments methodology. the formulated consortium-avs decolorized acid blue 15 and methylene blue with a higher average decolorization rate, which is more rapid than that of the pure cultures. the uv–vis spectrophotometric, fourier transform infra red spectrophotometric and high performance liquid chromatographic analysis confirm that the decolorization was due to biodegradation by oxido-reductive enzymes, produced by the consortium-avs. the toxicological assessment of plant growth parameters and the chlorophyll pigment concentrations of phaseolus mungo and triticum aestivum seedlings revealed the reduced toxic nature of the biodegraded products.