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  • enzymatic analyses demonstrate thermal adaptation of α-glucosidase activity in starch amended gully waste

    نویسندگان :
    جزئیات بیشتر مقاله
    • تاریخ ارائه: 1390/01/01
    • تاریخ انتشار در تی پی بین: 1390/01/01
    • تعداد بازدید: 672
    • تعداد پرسش و پاسخ ها: 0
    • شماره تماس دبیرخانه رویداد: -
     in this study we investigated the effect of starch amendment on α-glucosidase activity in an organic waste environment, treated under both mesophilic and thermophilic conditions. the relative effects of temperature on α-glucosidase activity with regard to in situ and assay conditions were investigated under a reciprocal design. the decline in dry matter under the different thermal regimes was consistent with the temperature independent degradation of starch. the results of extra-cellular enzyme analysis showed a significant relationship between starch addition and α-glucosidase activity, with evidence of thermal adaptation to the in situ temperature. a weaker, but significant, effect of starch addition on β-glucosidase activity was observed, with no evidence for thermal adaptation. thus, our data is consistent with a substrate adaptive response to temperature, albeit under potentially a high selective pressure.

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