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  • efficient production of l-asparaginase from bacillus licheniformis with low-glutaminase activity: optimization, scale up and acrylamide degradation studies

    جزئیات بیشتر مقاله
    • تاریخ ارائه: 1390/01/01
    • تاریخ انتشار در تی پی بین: 1390/01/01
    • تعداد بازدید: 596
    • تعداد پرسش و پاسخ ها: 0
    • شماره تماس دبیرخانه رویداد: -
     l-asparaginase has potential as an anti-cancer drug and for prevention of acrylamide formation in fried and baked foods. production of the enzyme by bacillus licheniformis(ram-8) was optimized by process engineering using a statistical modeling approach and a maximum yield of 32.26 iu/ml was achieved. the l-asparaginase exhibited glutaminase activity of only 0.8 iu/ml and would therefore be less prone to cause the side effects associated with asparaginase therapy compared to enzyme preparations with higher glutaminase activities. when production was carried out in a 30-l bioreactor, enzyme production reached 29.94 iu/ml in 15 h. the enzyme inhibited poly-acrylamide formation in 10% acrylamide solution and reduced acrylamide formation in fried potatoes by 80%.

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