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  • overexpression of a paenibacillus campinasensis xylanase in bacillus megaterium and its applications to biobleaching of cotton stalk pulp and saccharification of recycled paper sludge

    جزئیات بیشتر مقاله
    • تاریخ ارائه: 1390/01/01
    • تاریخ انتشار در تی پی بین: 1390/01/01
    • تعداد بازدید: 521
    • تعداد پرسش و پاسخ ها: 0
    • شماره تماس دبیرخانه رویداد: -
     a xylanase gene (xyng1-1) from paenibacillus campinasensis g1-1 was expressed inbacillus megaterium ms941 and a high level of extracellular xylansae activity (304.26 iu/ml) was achieved after induction with 0.5% xylose. the purified recombinant xylanase (xyng1-1r) revealed optimal activity at 60 °c and ph 7.0 and retained 79% and 81% activity after incubation without substrate at 60 °c, ph 5.0 and ph 8.0 for 3 h, respectively. application of xyng1-1r (15 iu/g pulp) to cotton stalk pulp bleaching increased brightness by 3.65% over that of the control without the xylanase and reduced the need for chlorine compounds by 50% without loss of brightness and pulp fiber qualities. when xyng1-1r (80 iu/g paper sludge) was used in combination with mixed cellulolytic enzymes, the saccharification efficiency of recycled paper sludge was increased by 10%. these results indicated that xyng1-1r is a promising candidate for various industrial applications such as biobleaching and bioenergy conversion.

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