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  • immobilization of laccase on magnetic bimodal mesoporous carbon and the application in the removal of phenolic compounds

    جزئیات بیشتر مقاله
    • تاریخ ارائه: 1390/01/01
    • تاریخ انتشار در تی پی بین: 1390/01/01
    • تعداد بازدید: 946
    • تعداد پرسش و پاسخ ها: 0
    • شماره تماس دبیرخانه رویداد: -
     a novel magnetically separable laccase immobilized system was constructed by adsorbing laccase into bimodal carbon-based mesoporous magnetic composites (cmmc). a large adsorption capacity (491.7 mg g−1), excellent activity recovery (91.0%) and broader ph and temperature profiles than free laccase have been exhibited by the immobilized laccase. thermal stability was enhanced to a great extent and operational stability was increased to a certain extent. the shift of kinetic parameters indicated affinity change between enzyme and substrate. application of the immobilized system in phenol and p-chlorophenol removal was investigated in a batch system. adsorption effects of the support were responsible for the quick removal rate in the first hour, and up to 78% and 84% of phenol and p-chlorophenol were removed in the end of the reaction, respectively, indicating that the magnetic bimodal mesoporous carbon is a promising carrier for both immobilization of laccase and further application in phenol removal.

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