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نویسندگان مقالات پربازدید :
فناوری منابع زیستی
جزئیات رویداد
اینستاگرام تی پی بین
همایش ، رویداد ، ژورنال
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  • one-step enzymatic synthesis of nucleosides from low water-soluble purine bases in non-conventional media

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    • تاریخ ارائه: 1390/01/01
    • تاریخ انتشار در تی پی بین: 1390/01/01
    • تعداد بازدید: 647
    • تعداد پرسش و پاسخ ها: 0
    • شماره تماس دبیرخانه رویداد: -
     the effect of several water-miscible cosolvents on activity and stability of soluble and immobilized 2′-deoxyribosyltransferase from lactobacillus reuteri on sepabeads® has been studied in order to establish optimal conditions for enzymatic synthesis of nucleosides using purine bases with low solubility in aqueous buffer. as a rule of thumb, there was a general reduction of soluble enzyme activity when cosolvent content was gradually increased in reaction medium. in contrast, immobilized enzyme activity was enhanced 1.2–1.4-fold at 20% of methanol, ethanol, 2-propanol, diethylene glycol, and acetone; and at 10% and 30% acetonitrile. likewise, highest increased activity (1.8-fold) was also obtained in presence of 20% acetonitrile. immobilized enzyme was successfully used in the synthesis of 2′-deoxyxanthosine and 2′-deoxyguanosine using 2′-deoxyuridine as sugar donor and the corresponding poor water-soluble base in the presence of 30% of methanol, ethanol, 2-propanol, ethylene glycol, acetonitrile, and dmso, giving high nucleoside yields at 4 h.

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