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  • sm-like protein enhanced tolerance of recombinant saccharomyces cerevisiae to inhibitors in hemicellulosic hydrolysate

    نویسندگان :
    جزئیات بیشتر مقاله
    • تاریخ ارائه: 1390/01/01
    • تاریخ انتشار در تی پی بین: 1390/01/01
    • تعداد بازدید: 543
    • تعداد پرسش و پاسخ ها: 0
    • شماره تماس دبیرخانه رویداد: -
     a current challenge of the cellulosic ethanol industry is to improve the resistance of inhibitors present in biomass hydrolysates. rna-binding protein gene lsm6 was cloned from industrial saccharomyces cerevisiae zu-e8, which is able to conferment glucose and xylose, and transformed into zu-e8 via expression vector prs426. the positive transformant zu-910 with over-expressing lsm6 was identified on the culture plates using high concentration of acetate and re-screened by fermentation test. fermentation by the recombinants was performed in a medium containing 80 g/l xylose and 2 g/l acetic acid or 20 g/l nh4ac/naac. after 96 h shaking-flask fermentation, zu-910 utilized 90.2% xylose with an ethanol yield of 26.9 g/l, which was 8.5- and 10-fold higher than zu-e8. further, in the corn stover hemicellulosic hydrolysate fermentation, both the xylose conversion and ethanol production by zu-910 was larger by 50% and 40% than zu-e8. zu-910 has also enhanced tolerance against furfural and so42−.

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