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فناوری منابع زیستی
جزئیات رویداد
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همایش ، رویداد ، ژورنال
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  • biocatalytic production of novel glycolipids with cellodextrin phosphorylase

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    • تاریخ ارائه: 1390/01/01
    • تاریخ انتشار در تی پی بین: 1390/01/01
    • تعداد بازدید: 585
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     glycolipids have gained increasing attention as natural surfactants with a beneficial environmental profile. they are typically produced by fermentation, which only gives access to a limited number of structures. here we describe the biocatalytic production of novel glycolipids with the cellodextrin phosphorylase from clostridium stercorarium. this enzyme was found to display a broad donor and acceptor specificity, allowing the synthesis of five different products. indeed, using either α-glucose 1-phosphate or α-galactose 1-phosphate as glycosyl donor, sophorolipid as well as glucolipid could be efficiently glycosylated. the transfer of a glucosyl moiety afforded a mixture of products that precipitated from the solution, resulting in near quantitative yields. the transfer of a galactosyl moiety, in contrast, generated a single product that remained in solution at thermodynamic equilibrium. these glycolipids not only serve as a new class of biosurfactants, but could also have applications in the pharmaceutical and nanomaterials industries.

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