Background & Aims: Hemophilia A is a common disorder of blood coagulation caused by deficiency of factor VIII with X-linked recessive inheritance affecting approximately 1 in 5000-10,000 male births worldwide. Hemophilia A caused by a heterogeneous spectrum of molecular defects in factor VIII gene .The most common mutation is the intron 22 inversion which can be analyzed by several methods, such as; Southern blotting , LD PCR or IS-PCR. This study aimed to identify the prevalence of intron 2...