مقاله Modeling of equilibrium and kinetics of human polyclonal immunoglobulin G adsorption on a tentacle cation exchanger : تی پی بین

خلاصه مقاله

Adsorption of human immunoglobulin G (IgG) on a commercial cation exchanger with a grafted polymer layer was investigated at pH 4.5 and in the NaCl concentration range of 0–150 mM. Adsorption equilibrium was determined in static batch experiments and verified in batch uptake experiments. Parameters of the Langmuir isotherm were estimated for each salt concentration separately. The batch uptake experiments provided also the estimates of effective pore diffusion coefficients of IgG for individual protein and salt concentrations. The values of the effective pore diffusion coefficient depended strongly on both factors. They increased by about 5–15 times with the NaCl concentration and decreased about three times with the protein concentration. The quality of the estimated parameters was confirmed by frontal experiments described by the general rate model of chromatography.

نویسندگان

Katarzyna Wrzosek [ Department of Chemical and Biochemical Engineering, Institute of Chemical and Environmental Engineering, Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava, Radlinského 9, 812 37, Bratislava, Slovakia ]

Pavel Ačai [ Department of Chemical and Biochemical Engineering, Institute of Chemical and Environmental Engineering, Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava, Radlinského 9, 812 37, Bratislava, Slovakia ]

Michal Gramblička [ Department of Chemical and Biochemical Engineering, Institute of Chemical and Environmental Engineering, Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava, Radlinského 9, 812 37, Bratislava, Slovakia ]

Milan Polakovič [ Department of Chemical and Biochemical Engineering, Institute of Chemical and Environmental Engineering, Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava, Radlinského 9, 812 37, Bratislava, Slovakia ]

کلمات کلیدی

cation exchange chromatography grafted tentacle layer IgG adsorption Langmuir isotherm effective pore diffusion coefficient general rate model

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Chemical Papers 2013

Chemical Papers is devoted to research in pure and applied chemistry and chemical engineering. At its founding in 1947, the journal presented the achievements of Slovak chemical science. Today, it is a peer-reviewed international journal published in English by the Institute of Chemistry of the Slovak Academy of Sciences, with the support of several other academic institutions. Its interdisciplinary coverage brings chemistry research together with related disciplines, including analytical chemistry; (bio)catalysis; biochemistry; chemical (bio)technology; (bio)chemical engineering; chemical physics; drug chemistry; food chemistry; environmental chemistry; inorganic chemistry; macromolecular chemistry; materials chemistry; mathematical chemistry; medical and pharmaceutical chemistry; nanochemistry; organic chemistry; physical chemistry and more.

حوزه های تحت پوشش : Chemistry/Food Science, general, Industrial Chemistry/Chemical Engineering, Biochemistry, generalBiotechnology