محبوبترین نویسندگان :
نویسندگان مقالات پربازدید :
بیوشیمی مولکولی و سلولی 2013
جزئیات رویداد
همایش ، رویداد ، ژورنال
اینستاگرام تی پی بین
حوزه های تحت پوشش رویداد

  • prognostic significance of microrna-141 expression and its tumor suppressor function in human pancreatic ductal adenocarcinoma

    نویسندگان :
    کلمات کلیدی :
    جزئیات بیشتر مقاله
    • تاریخ ارائه: 1392/07/24
    • تاریخ انتشار در تی پی بین: 1392/07/24
    • تعداد بازدید: 884
    • تعداد پرسش و پاسخ ها: 0
    • شماره تماس دبیرخانه رویداد: -
     increasing evidence shows that dysregulation of micrornas is correlated with tumor development. this study was performed to determine the expression of mir-141 and investigate its clinical significance in pancreatic ductal adenocarcinoma (pdac). taqman quantitative rt-pcr was used to detect mir-141 expressions in 94 pdac tissues and 16 nontumorous pancreatic tissues. correlations between mir-141 expression and clinicopathologic features and prognosis of patients were statistically analyzed. the effects of mir-141 expression on growth and apoptosis of pdac cell line (panc-1) were determined by mtt, colony formation, and flow cytometry assays. potential target genes were identified by luciferase reporter and western blot assays. the expression level of mir-141 in pdac tissues was significantly lower than that in corresponding nontumorous tissues. downregulation of mir-141 correlated with poorer pt and pn status, advanced clinical stage, and lymphatic invasion. also, low mir-141 expression in pdac tissues was significantly correlated with shorter overall survival, and multivariate analysis showed that mir-141 was an independent prognostic factor for pdac patients. further, functional researches suggested that mir-141 inhibits growth and colony formation, and enhances caspase-3-dependent apoptosis in panc-1 cells by targeting yes-associated protein-1 (yap1). therefore, mir-141 is an independent prognostic factor for pdac patients, and functions as a tumor suppressor gene by targeting yap1.

سوال خود را در مورد این مقاله مطرح نمایید :

با انتخاب دکمه ثبت پرسش، موافقت خود را با قوانین انتشار محتوا در وبسایت تی پی بین اعلام می کنم
مقالات جدیدترین رویدادها
مقالات جدیدترین ژورنال ها