محبوبترین نویسندگان :
نویسندگان مقالات پربازدید :
بیوشیمی مولکولی و سلولی 2013
جزئیات رویداد
همایش ، رویداد ، ژورنال
اینستاگرام تی پی بین
حوزه های تحت پوشش رویداد

  • silymarin- and melatonin-mediated changes in the expression of selected genes in pesticides-induced parkinsonism

    نویسندگان :
    کلمات کلیدی :
    جزئیات بیشتر مقاله
    • تاریخ ارائه: 1392/07/24
    • تاریخ انتشار در تی پی بین: 1392/07/24
    • تعداد بازدید: 933
    • تعداد پرسش و پاسخ ها: 0
    • شماره تماس دبیرخانه رویداد: -
     parkinson’s disease (pd) is the second most unconcealed neurodegenerative disorder labelled with motor impairments. two pesticides, manganese ethylene-1,2-bisdithiocarbamate (maneb) and 1,1′-dimethyl-4,4′-bipyridinium dichloride (paraquat), together, are reported to increase the incidence of pd in humans and parkinsonism in mice. conversely, silymarin and melatonin, two naturally occurring antioxidants, rescue from maneb- and paraquat-induced parkinsonism. the study examined silymarin- and melatonin-mediated changes in the expression of selected genes in maneb- and paraquat-induced parkinsonism employing mouse discover chips microarrays. the mice were treated intraperitoneally (i.p.), daily, with silymarin (40 mg/kg) or melatonin (30 mg/kg) for 9 weeks along with vehicles. subsets of animals were also treated with maneb (30 mg/kg; i.p.) and paraquat (10 mg/kg; i.p.), twice a week, for 9 weeks. whilst the expression of genes in the striatum was determined by microarray, the expression of randomly selected transcripts was validated by quantitative real-time polymerase chain reaction (qrt-pcr). combined maneb- and paraquat-treatment altered the expression of several genes associated with apoptosis, inflammation, cell cycle, cell-signalling, etc. pathways. silymarin and melatonin significantly resisted the changes in the expression of a few genes related to apoptosis, inflammation, cell cycle, cell-signalling, etc. the expression patterns of seven randomly selected genes were analyzed by qrt-pcr, which were found to follow the similar trends, as observed with microarray. the results obtained from the study thus demonstrate that despite resemblances, silymarin and melatonin differentially offset maneb- and paraquat-induced changes in transcriptome.

سوال خود را در مورد این مقاله مطرح نمایید :

با انتخاب دکمه ثبت پرسش، موافقت خود را با قوانین انتشار محتوا در وبسایت تی پی بین اعلام می کنم
مقالات جدیدترین رویدادها
مقالات جدیدترین ژورنال ها