مقاله Do Ca2+-chelating polysaccharides reduce calcium ion release from gypsum-based biomaterials? : تی پی بین

خلاصه مقاله

Background

Calcium sulphate, a widely used bone filler, may negatively affect human osteoblasts due to release of high quantities of calcium ions. To reduce this effect, an attempt was made to enrich calcium sulphate with Ca2+-chelating plant and rhizobial exopolysaccharides (EPS).

Methodology

Incubation of polysaccharide-enriched calcium sulphate composites was performed in DMEM/F12 medium. Ca2+ (and Mg2+ and Pi) levels were estimated using standardised, spectrophotometry-based kits. Composite surface morphology was tested using SEM technique.

Results

Rhizobial EPS was found slightly less effective at Ca2+ chelation than sodium alginate. Both polysaccharides may be used as gypsum supplements in the form of setting liquids (0.3% total mass), but only sodium alginate may be used as a powder (up to 5% total mass of the composite). Polysaccharide-triggered reduction of Ca2+ release reached the level of 50% during the first 2.5 h of incubation, then decreased significantly.

Conclusions

Both tested polysaccharides possess calcium-chelating properties. However, although alginate caused a reduction in Ca2+ levels in the media incubated with the gypsum samples, the reduction was too short lived to provide a long-term effect. Further modification of the composite content using calcium-deficient hydroxyapatite and low-molecular weight rhizobial EPS with higher solubility could bring more satisfactory results.

نویسندگان

Anna Belcarz [ Chair and Department of Biochemistry and Biotechnology, Medical University in Lublin, 20-093, Lublin, Poland ]

Monika Janczarek [ Department of Genetics and Microbiology, University of M. Curie-Skłodowska, 20-033, Lublin, Poland ]

Karolina Kolacz [ Chair and Department of Biochemistry and Biotechnology, Medical University in Lublin, 20-093, Lublin, Poland ]

Teresa Urbanik-Sypniewska [ Department of Genetics and Microbiology, University of M. Curie-Skłodowska, 20-033, Lublin, Poland ]

Grazyna Ginalska [ Chair and Department of Biochemistry and Biotechnology, Medical University in Lublin, 20-093, Lublin, Poland ]

کلمات کلیدی

Rhizobial exopolysaccharides Alginate Calcium ion release Ion uptake Gypsum Implantable materials

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Central European Journal of Biology 2014

Central European Journal of Biology (CEJB) is an English-language journal devoted to rapid publication of original research and review articles in all biology fields. The aim of this journal is to become the premier source of high quality research from Central and Eastern Europe; however, contributions are welcome from all over the world. The journal is published bimonthly. Central European Journal of Biology presents rigorously peer-reviewed scientific articles in the following categories: Research Articles, Communications, Reviews and Mini-Reviews, Commentaries and Letters to the Editor. Coverage includes Cellular and Molecular Life Sciences, Biomedical Sciences, Plant Sciences, Animal Sciences and Evolution, Ecology Environment. The aim of the editors is to publish timely, useful and informative results that will be of value to a general biological audience. We provide free language assistance to the authors of all accepted articles. Every article published in our journal is promoted to researchers working in the respective field.

حوزه های تحت پوشش : Life Sciences, general, Cell Biology, Biochemistry, general, Human Genetics, Immunology