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مجله زیست شناسی اروپای مرکزی 2014
جزئیات رویداد
همایش ، رویداد ، ژورنال
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    • تاریخ ارائه: 1392/07/24
    • تاریخ انتشار در تی پی بین: 1392/07/24
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    • شماره تماس دبیرخانه رویداد: -
     we hypothesized that the mechanism responsible for the anti-atherosclerotic action of bilberry extract (be) is linked to its antioxidant and anti-inflammatory potential, and investigated its direct effect on the regulation of apolipoprotein e (apoe) and cholesteryl ester transfer protein (cetp) secretion from lipid-loaded macrophages. human thp-1 macrophages were loaded with lipids by incubation with human copper-oxidized ldl (oxldl) and then exposed to different concentrations of be (1–5 µg ml−1) obtained from bilberries (mechanically homogenized and solubilized in ethanol). cellular and secreted proteins, the phosphorylation level of nf-κb and protein kinase a (pka) were quantified by western blot and gene expression was evaluated by real-time pcr. the results showed that be induced in lipid-loaded macrophages has: (i) an antioxidant effect by reducing the expression of nadph oxidase subunits, p22phox, p47phox and nox4, (ii) an anti-inflammatory effect by diminishing the secretion of crp, mcp-1 and il-1β and (iii) cholesterol efflux by increasing the secretion of apoe and cetp and by reducing cellular cholesterol content. be exerted these effects by inhibition of nf-κb and activation of pka signaling pathways. our study supports be therapeutic administration to decrease oxidative and inflammatory stress by a molecular mechanism regulated by nf-κb and pka signaling pathways in lipid-loaded macrophages.

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