مقاله ECM spreading behaviour on micropatterned TiO2 nanotube surfaces : تی پی بین

خلاصه مقاله

By electrochemical anodization, highly ordered nanotubular TiO₂ structures were formed on titanium surfaces with diameters of 15 and 100 nm. In previous work we showed that 15 nm tubes strongly enhanced adhesion and vitality of many cell types, whereas on 100 nm diameter tubes the induction of apoptosis was observed. In the present work we produce mixed (15 nm contrasted with 100 nm) nanotube microstructures that combine highly defined micro- and nanostructures using a photolithographic approach to achieve a direct comparison of adhesion and spreading of mesenchymal stem cells on different diameter nanotubes present on a single surface. On these coupled different nanoscale surfaces mesenchymal stem cell adhesion is initially favoured on 15 nm tube areas but, with time, a gradient in cell number and shape to the “unfavourable” regions of the substrate (100 nm tubes) can be observed. This can be explained by cells on the “favourable” 15 nm regions that strongly produce and shed extracellular matrix onto the “unfavourable” locations. These findings contribute to the design of cell guiding surfaces, but also demonstrate the need for a long-range defined homogeneous order when studying cell behaviour on nanostructured surfaces.

نویسندگان

Andreas Pittrof [ Department of Materials Science, WW4-LKO, University of Erlangen-Nuremberg, Martensstrasse 7, 91058 Erlangen, Germany ]

Jung Park [ Department of Experimental Medicine I, Nikolaus Fiebiger-Center of Molecular Medicine, Glueckstrasse 6, 91054 Erlangen, Germany ]

Sebastian Bauer [ Department of Materials Science, WW4-LKO, University of Erlangen-Nuremberg, Martensstrasse 7, 91058 Erlangen, Germany ]

Patrik Schmuki [ Department of Materials Science, WW4-LKO, University of Erlangen-Nuremberg, Martensstrasse 7, 91058 Erlangen, Germany ]

کلمات کلیدی

TiO2 nanotubes Mesenchymal stem cells ECM Micropatterning Photolithography

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Acta Biomaterialia

Acta Biomaterialia is an international journal that publishes peer-reviewed original research reports, review papers and communications in the broadly defined field of biomaterials science. The emphasis of the journal is on the relationship between biomaterial structure and function at all length scales. The journal is committed to rapid peer-review and publication.

حوزه های تحت پوشش : biomaterials science