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  • batch- and continuous propionic acid production from glycerol using free and immobilized cells of propionibacterium acidipropionici

    جزئیات بیشتر مقاله
    • تاریخ ارائه: 1390/01/01
    • تاریخ انتشار در تی پی بین: 1390/01/01
    • تعداد بازدید: 600
    • تعداد پرسش و پاسخ ها: 0
    • شماره تماس دبیرخانه رویداد: -
     propionic acid production from glycerol was studied using propionibacterium acidipropionici dsm 4900 cells immobilized on polyethylenimine-treated poraver (pei-poraver) and luffa (pei-luffa), respectively. using pei-luffa, the average productivity, yield and concentration of propionic acid from 40 g l-1 glycerol were 0.29 g l-1 h-1, 0.74 molpa molgly-1 and 20 g l-1, respectively, after four consecutive recycle-batches. pei-poraver supported attachment of 31 times higher amounts of cells than pei-luffa and produced 20, 28 and 35 g l-1 propionic acid from 40, 65 and 85 g l-1 glycerol, respectively (0:61 molpa mol-1glyþ. the corresponding production rates were 0.86, 0.43 and 0.35 g l-1 h-1, which are the highest reported from glycerol via batch or fed-batch fermentations for equivalent propionic acid concentrations. using a continuous mode of operation at a dilution rate of 0.1 h-1, cell washout was observed in the bioreactor with free cells; however, propionic acid productivity, yield and concentration were 1.40 g l-1 h-1, 0:86 molpa mol-1gly, and 15 g l-1, respectively, using immobilized cells in the pei-poraver bioreactor. the choice of the immobilization matrix can thus significantly influence the fermentation efficiency and profile. the bioreactor using cells immobilized on pei-poraver allowed the fermentation of higher glycerol concentrations and provided stable and higher fermentation rates than that using free cells or the cells immobilized on pei-luffa.

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