مقاله Batch- and continuous propionic acid production from glycerol using free and immobilized cells of Propionibacterium acidipropionici : تی پی بین

خلاصه مقاله

Propionic acid production from glycerol was studied using Propionibacterium acidipropionici DSM 4900 cells immobilized on polyethylenimine-treated Poraver (PEI-Poraver) and Luffa (PEI-Luffa), respectively. Using PEI-Luffa, the average productivity, yield and concentration of propionic acid from 40 g L^-1 _glycerol were 0.29 g L^-1 h^-1, 0.74 mol_PA mol_Gly^-1 and 20 g L^-1, respectively, after four consecutive recycle-batches. PEI-Poraver supported attachment of 31 times higher amounts of cells than PEI-Luffa and produced 20, 28 and 35 g L^-1 propionic acid from 40, 65 and 85 g L^-1 glycerol, respectively (0:61 mol_PA mol^-1_GlyÞ. The corresponding production rates were 0.86, 0.43 and 0.35 g L^-1 h^-1, which are the highest reported from glycerol via batch or fed-batch fermentations for equivalent propionic acid concentrations. Using a continuous mode of operation at a dilution rate of 0.1 h^-1, cell washout was observed in the bioreactor with free cells; however, propionic acid productivity, yield and concentration were 1.40 g L^-1 h^-1, 0:86 mol_PA mol^-1_Gly, and 15 g L^-1, respectively, using immobilized cells in the PEI-Poraver bioreactor. The choice of the immobilization matrix can thus significantly influence the fermentation efficiency and profile. The bioreactor using cells immobilized on PEI-Poraver allowed the fermentation of higher glycerol concentrations and provided stable and higher fermentation rates than that using free cells or the cells immobilized on PEI-Luffa.

نویسندگان

Tarek Dishisha [ Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden ]

Maria Teresa Alvarez [ Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden ]

Rajni Hatti-Kaul [ Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden ]

کلمات کلیدی

Fermentation Platform chemical Organic acid Cell adsorption Polyethylenimine

آدرس این مقاله

آدرس مستقیم دسترسی به این مقاله

مشخصات کنفرانس

مشاهده مقالات

Bioresource Technology

The journal's aim is to advance and disseminate knowledge in all the related areas of biomass, biological waste treatment, bioenergy, biotransformations and bioresource systems analysis, and technologies associated with conversion or production.

حوزه های تحت پوشش : Bioresource Technology