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  • an antioxidant exopolysaccharide devoid of pro-oxidant activity produced by the soil bacterium bordetella sp. b4

    جزئیات بیشتر مقاله
    • تاریخ ارائه: 1390/01/01
    • تاریخ انتشار در تی پی بین: 1390/01/01
    • تعداد بازدید: 570
    • تعداد پرسش و پاسخ ها: 0
    • شماره تماس دبیرخانه رویداد: -
     an exopolysaccharide (eps) with a molecular weight of 230 kda, was isolated frombordetella sp. b4. the eps was identified as linear alpha-1,6-(6-methyl)-glucan with n-acetyl-d-glucosamine branches at alpha-1, 4-linkages by ir and nmr spectroscopy. the free radical scavenging capacities of eps on 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (abts+), h2o2, −oh and lipid peroxidation were 2-, 86-, 134- and 18-fold higher than that of ascorbic acid, respectively. compared with ascorbic acid, the eps was more effective in preventing dna and protein from free radical damage induced by 2,2′-azobis (2-amidinopropane) dihydrochloride (aaph). more significantly, the eps did not degrade dna and protein by the pro-oxidant effect in the presence of copper ions and h2o2. furthermore, eps could protect human umbilical vein endothelium cells (huvecs) from high glucose-mediated damage. the production of eps reached 10.2 g/l in the fermentation medium containing 3.0 g/l cholesterol, suggesting thatbordetella sp. b4 was a potential producer of antioxidant eps.

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