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آکتا بیومتریال
جزئیات رویداد
همایش ، رویداد ، ژورنال
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  • thick collagen-based 3d matrices including growth factors to induce neurite outgrowth

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    • تاریخ ارائه: 1392/01/01
    • تاریخ انتشار در تی پی بین: 1392/01/01
    • تعداد بازدید: 629
    • تعداد پرسش و پاسخ ها: 0
    • شماره تماس دبیرخانه رویداد: -
     designing synthetic microenvironments for cellular investigations is a very active area of research at the crossroads of cell biology and materials science. the present work describes the design and functionalization of a three-dimensional (3d) culture support dedicated to the study of neurite outgrowth from neural cells. it is based on a dense self-assembled collagen matrix stabilized by 100-nm-wide interconnected native fibrils without chemical crosslinking. the matrices were made suitable for cell manipulation and direct observation in confocal microscopy by anchoring them to traditional glass supports with a calibrated thickness of ∼50 μm. the matrix composition can be readily adapted to specific neural cell types, notably by incorporating appropriate neurotrophic growth factors. both pc-12 and sh-sy5y lines respond to growth factors (nerve growth factor and brain-derived neurotrophic factor, respectively) impregnated and slowly released from the support. significant neurite outgrowth is reported for a large proportion of cells, up to 66% for pc12 and 49% for sh-sy5y. it is also shown that both growth factors can be chemically conjugated (edc/nhs) throughout the matrix and yield similar proportions of cells with longer neurites (61% and 52%, respectively). finally, neurite outgrowth was observed over several tens of microns within the 3d matrix, with both diffusing and immobilized growth factors.

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