اینستاگرام تی پی بین
همایش ، رویداد ، ژورنال
حوزه های تحت پوشش رویداد
  • preparatory production of quercetin-3-β-d-glucopyranoside using alkali-tolerant thermostable α-l-rhamnosidase from aspergillus terreus

    جزئیات بیشتر مقاله
    • تاریخ ارائه: 1390/01/01
    • تاریخ انتشار در تی پی بین: 1390/01/01
    • تعداد بازدید: 564
    • تعداد پرسش و پاسخ ها: 0
    • شماره تماس دبیرخانه رویداد: -
     extensive screening for a robust producer of α-l-rhamnosidase activity from well-defined strains of filamentous fungi, including multifactorial optimization (inducers, cultivation conditions) was accomplished. enzyme production of the optimal producer aspergillus terreus (non-toxigenic) was scaled up to 50 l. α-l-rhamnosidase, which was fully characterized, proved to be thermo- and alkali-tolerant, thus enabling effective operation at 70 °c and ph 8.0. these conditions allow for a very high substrate (rutin) load up to 100–300 g/l, thus enabling very high volumetric productivity of the reaction product quercetin-3-β-d-glucopyranoside (isoquercitrin). here, a novel concept of “immobilised substrate” is used. isoquercitrin is a highly effective and biocompatible antioxidant with strong anti-inflammatory activities. rutin biotransformation was optimized and scaled up to ca 10 kg production and thus the robustness of the large-scale production was demonstrated. isoquercitrin can be produced to a very high purity (98%) in multikilogram amounts, without any quercetin and directly applicable in nutraceuticals.

سوال خود را در مورد این مقاله مطرح نمایید :

با انتخاب دکمه ثبت پرسش، موافقت خود را با قوانین انتشار محتوا در وبسایت تی پی بین اعلام می کنم
مقالات جدیدترین رویدادها
مقالات جدیدترین ژورنال ها