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  • fed-batch fermentation and supercritical fluid extraction of heterotrophic microalgal chlorella protothecoides lipids

    نویسندگان :
    جزئیات بیشتر مقاله
    • تاریخ ارائه: 1390/01/01
    • تاریخ انتشار در تی پی بین: 1390/01/01
    • تعداد بازدید: 627
    • تعداد پرسش و پاسخ ها: 0
    • شماره تماس دبیرخانه رویداد: -
     lipids obtained from chlorella protothecoides in heterotrophic cultivation are considered a suitable feedstock for biodiesel production. in this study, glucose fed-batch fermentation was performed to increase final biomass and lipid production. the biomass productivity and lipid productivity were 6.28 and 2.06 g/l day, respectively. biomass/glucose conversion and the lipid/glucose conversion were 43.3% and 14.2%, respectively. extraction of lipids from algae has been identified as a key bottleneck in bioprocessing operations. supercritical carbon dioxide (sc-co2) was applied for neutral lipids extraction and the sc-co2 kinetics was investigated by the goto et al. model. the modeling showed a good fit with experimental data. additionally, neutral lipids extracted by sc-co2 displayed a suitable fatty acid profile for biodiesel [mainly c18:1 (60.0%), c18:2 (18.7%) and c16:0 (11.5%)]. our study demonstrated the ability to produce high levels of neutral lipids through heterotrophic algal culture and subsequent extraction of lipids with sc-co2 method developed.

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