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  • evaluation of by-products from the biodiesel industry as fermentation feedstock for poly(3-hydroxybutyrate-co-3-hydroxyvalerate) production by cupriavidus necator

    جزئیات بیشتر مقاله
    • تاریخ ارائه: 1390/01/01
    • تاریخ انتشار در تی پی بین: 1390/01/01
    • تعداد بازدید: 519
    • تعداد پرسش و پاسخ ها: 0
    • شماره تماس دبیرخانه رویداد: -
     utilization of by-products from oilseed-based biodiesel production (crude glycerol, rapeseed meal hydrolysates) for microbial polyhydroxyalkanoate (pha) production could lead to the replacement of expensive carbon sources, nutrient supplements and precursors for co-polymer production. batch fermentations in shake flasks with varying amounts of free amino nitrogen led to the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (p(3hb-co-3hv)) with a 2.8–8% 3hv content. fed-batch fermentations in shake flasks led to the production of 10.9 g/l p(3hb-co-3hv) and a 55.6% p(3hb-co-3hv) content. nacl concentrations between 2 and 6 g/l gradually became inhibitory to bacterial growth and pha formation, whereas in the case of k2so4, the inhibitory effect was observed only at concentrations higher than 20 g/l. differential scanning calorimetry (dsc), thermogravimetric analysis (tga) and nuclear magnetic resonance (13c nmr) demonstrated that the incorporation of 3hv into the obtained p(3hb-co-3hv) lowered glass transition temperature, crystallinity and melting point as compared to polyhydroxybutyrate. integrating pha production in existing oilseed-based biodiesel plants could enhance the viability and sustainability of this first generation biorefinery.

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