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  • bioremediation of waste cooking oil using a novel lipase produced by penicillium chrysogenum snp5 grown in solid medium containing waste grease

    جزئیات بیشتر مقاله
    • تاریخ ارائه: 1390/01/01
    • تاریخ انتشار در تی پی بین: 1390/01/01
    • تعداد بازدید: 774
    • تعداد پرسش و پاسخ ها: 0
    • شماره تماس دبیرخانه رویداد: -
     the aim of present work was to bioremediate the waste cooking oil using a novel lipase produced in solid medium containing waste grease and wheat bran by penicillium chrysogenum. enzyme extracted with phosphate buffer was purified 10.6 and 26.28-fold after 90% ammonium sulfate precipitation and ion-exchange chromatography, respectively. the partial characterization of enzyme revealed its km and vmax value for p-nitrophenolpamitate as 0.4 mm and 47.61 u/ml, respectively. the relative molecular mass of lipase was 40 kda by sds–page and confirmed by zymogram. purified lipase was most stable at 40 °c and at 8.0 ph. lipase activity was enhanced by metal ions such as mg2+, fe2+, ca2+ and non-ionic surfactant tritonx-100, while suppressed in the presence of sds. crude lipase was applied on cooking oil waste and the acid value was 26.92 mg/g. this showed that the enzyme could be employed for the bioremediation of used cooking oil.

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