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مجله زیست شناسی اروپای مرکزی 2014
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  • hes-1-targeting sirna inhibits the maturation of murine myeloid-derived dendritic cells

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    • تاریخ ارائه: 1392/07/24
    • تاریخ انتشار در تی پی بین: 1392/07/24
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     activation of notch by jagged-1 may plays a pivotal role in maturation of dendritic cells (dcs), but the mechanism has not been completely defined. in the present study, hes-1 (hairy/enhancer-of-split)-targeting sirna was used to confirm a role of jagged-1-notch signaling pathway activation in maturation of murine bone marrow-derived dcs and to search for a target that plays a critical role. the results showed that compared with the control, lipopolysaccharide or zymosan a groups, jagged-1 (a soluble jagged 1/fc chimera protein) effectively increased expression of hes-1 and deltex-1 mrna, which could be reversed by dapt (2, 4-diamino-5-phenylthiazole), a specific inhibitor of the notch signaling pathway. hes-1-targeting sirna could successfully down-regulate the endogenous hes-1 expression in the dcs. concurrently, a significant down-regulation of cd40, cd80, cd86 and mhc-ii expressions on the surface of the dcs was found with the reduction of il-12 yielded by the dcs. our results demonstrate that hes-1-targeting sirna can inhibit the maturation of the dcs induced by jagged-1, indicating hes-1 may be an important target of notch signaling mediating the maturation of dcs.

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