مقاله Mineral coatings modulate β-TCP stability and enable growth factor binding and release : تی پی بین

خلاصه مقاله

β-Tricalcium phosphate (β-TCP) is an attractive ceramic for bone tissue repair because of its similar composition to bone mineral and its osteoconductivity. However, compared with other ceramics β-TCP has a rapid and uncontrolled rate of degradation. In the current study β-TCP granules were mineral coated with the aim of influencing the dissolution rate of β-TCP, and also to use the coating as a carrier for controlled release of the growth factors recombinant human vascular endothelial growth factor (rhVEGF), modular VEGF peptide (mVEGF), and modular bone morphogenetic protein 2 peptide (mBMP2). The biomineral coatings were formed by heterogeneous nucleation in aqueous solution using simulated body fluid solutions with varying concentrations of bicarbonate (HCO₃). Our results demonstrate that we could coat β-TCP granules with mineral layers possessing different dissolution properties. The presence of a biomineral coating delays the dissolution rate of the β-TCP granules. As the carbonate (CO₃²⁻) content in the coating was increased the dissolution rate of the coated β-TCP also increased, but remained slower than the dissolution of uncoated β-TCP. In addition, we showed sustained release of multiple growth factors, with release kinetics that were controllable by varying the identity of the growth factor or the CO₃²⁻ content in the mineral coating. Released rhVEGF induced human umbilical vein endothelial cell (HUVEC) proliferation, and mVEGF enhanced migration of mouse embryonic endothelial cells in a scratch wound healing assay, indicating that each released growth factor was biologically active.

نویسندگان

Darilis Suárez-González [ Materials Science Program, University of Wisconsin, Madison, WI 53706, USA ]

Jae Sung Lee [ Department of Biomedical Engineering, University of Wisconsin, Madison, WI 53706, USA ]

Sheeny K. Lan Levengood [ Department of Biomedical Engineering, University of Wisconsin, Madison, WI 53706, USA ]

Ray Vanderby Jr. [ Materials Science Program, University of Wisconsin, Madison, WI 53706, USA ]

William L. Murphy [ Materials Science Program, University of Wisconsin, Madison, WI 53706, USA ]

کلمات کلیدی

β TCP Bone morphogenetic protein 2 Vascular endothelial growth factor Bone tissue engineering Hydroxyapatite

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Acta Biomaterialia

Acta Biomaterialia is an international journal that publishes peer-reviewed original research reports, review papers and communications in the broadly defined field of biomaterials science. The emphasis of the journal is on the relationship between biomaterial structure and function at all length scales. The journal is committed to rapid peer-review and publication.

حوزه های تحت پوشش : biomaterials science