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آکتا بیومتریال
جزئیات رویداد
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  • polymer micelles with pyridyl disulfide-coupled antigen travel through lymphatics and show enhanced cellular responses following immunization

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    • تاریخ ارائه: 1392/01/01
    • تاریخ انتشار در تی پی بین: 1392/01/01
    • تعداد بازدید: 720
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    • شماره تماس دبیرخانه رویداد: -
     poly(ethylene glycol)-stabilized poly(propylene sulfide) core (peg-pps) nanoparticles (nps) smaller than 50 nm efficiently travel to draining lymph nodes and interact with antigen-presenting cells (apcs) to induce potent immune responses following intradermal immunization. to determine if a similar system could be developed that could be more easily and reproducibly prepared and eliminated faster in vivo, we created block copolymers of peg-bl-pps capable of self-assembling into 25–35 nm micelles (mcs). biodistribution studies showed that these mcs were able to travel to draining lymph nodes, where they preferentially interacted with apcs. to couple cysteine-containing antigens to the surface of the mcs, a new polymer was synthesized with a terminal pyridyl disulfide (pds), forming pds-peg-bl-pps-benzyl. when mice were immunized in conjunction with free cpg as an adjuvant, ovalbumin-conjugated mcs (mc-ova) generated more (2.4-fold) ova-specific cd8+ t cells in the blood and higher (1.7-fold) interferon-gamma levels from splenocytes upon restimulation than in mice immunized with free ova and cpg. when comparing this mc platform to our peg-pps nps with disulfide-linked ova, no significant differences were found in the measured responses. these results indicate that pds-functionalized mcs are efficient antigen delivery vehicles that enhance immune responses compared to immunization with free protein.

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