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  • arabinose substitution degree in xylan positively affects lignocellulose enzymatic digestibility after various naoh/h2so4 pretreatments in miscanthus

    جزئیات بیشتر مقاله
    • تاریخ ارائه: 1390/01/01
    • تاریخ انتشار در تی پی بین: 1390/01/01
    • تعداد بازدید: 504
    • تعداد پرسش و پاسخ ها: 0
    • شماره تماس دبیرخانه رویداد: -
     xylans are the major hemicelluloses in grasses, but their effects on biomass saccharification remain unclear. in this study, we examined the 79 representativemiscanthus accessions that displayed a diverse cell wall composition and varied biomass digestibility. correlation analysis showed that hemicelluloses level has a strong positive effect on lignocellulose enzymatic digestion after naoh or h2so4 pretreatment. characterization of the monosaccharide compositions in the koh-extractable and non-koh-extractable hemicelluloses indicated that arabinose substitution degree of xylan is the key factor that positively affects biomass saccharification. the xylose/arabinose ratio after individual enzyme digestion revealed that the arabinose in xylan is partially associated with cellulose in the amorphous regions, which negatively affects cellulose crystallinity for high biomass digestibility. the results provide insights into the mechanism of lignocellulose enzymatic digestion upon pretreatment, and also suggest a goal for the genetic modification of hemicelluloses towards the bioenergy crop breeding ofmiscanthus and grasses.

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