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  • a new thermostable β-glucosidase mined from dictyoglomus thermophilum: properties and performance in octyl glucoside synthesis at high temperatures

    جزئیات بیشتر مقاله
    • تاریخ ارائه: 1390/01/01
    • تاریخ انتشار در تی پی بین: 1390/01/01
    • تعداد بازدید: 634
    • تعداد پرسش و پاسخ ها: 0
    • شماره تماس دبیرخانه رویداد: -
     a new β-glucosidase (dtgh) representing 40% identity with an apple seed glycosidase (asg) was cloned from dictyoglomus thermophilum. dtgh showed extremely high thermostability in aqueous solution, with half-lives of 533, 44, and 5 h measured at 70, 80 and 90 °c, respectively. therefore it was used for direct glycosylation of n-octanol at 70 °c instead of 50 °c as usually. as a result, the glucose based conversion was increased by 27%, but the time spent to reach equilibrium was decreased from 7 d to 3 d. this enzyme also exhibited excellent stability under the reaction environment, retaining 70–80% of its initial activity after 7 d of incubation at 70 °c in either 1.7 m glucose solution or octanol-aqueous (85:15, v/v) system. it could retain part of synthetic activity even in boiling water. owing to the strong glucose-tolerance and extremely high thermostability, dtgh should be promising for various glucosides synthesis.

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