effects of stress fiber contractility on uniaxial stretch guiding mitosis orientation and stress fiber alignment

it has been documented that mitosis orientation (mo) is guided by stress fibers (sfs), which are perpendicular to exogenous cyclic uniaxial stretch. however, the effect of mechanical forces on mo and the mechanism of stretch-induced sfs reorientation are not well elucidated to date. in the present study, we used murine 3t3 fibroblasts as a model, to investigate the effects of uniaxial stretch on sfo and mo utilizing custom-made stretch device. we found that cyclic uniaxial stretch induced both sfs and mitosis directions orienting perpendicularly to the stretch direction. the f-actin and myosin ii blockages, which resulted in disoriented sfs and mitosis directions under uniaxial stretch, suggested a high correlation between sfo and mo. y27632 (10 μm), ml7 (50 μm, or 75 μm), and blebbistatin (50 μm, or 75 μm) treatments resulted in sfo parallel to the principle stretch direction. upon stimulating and inhibiting the phosphorylation of myosin light chain (p-mlc), we observed a monotonic proportion of sfo to the level of p-mlc. these results suggested that the level of cell contraction is crucial to the response of sfs, either perpendicular or parallel, to the external stretch. showing the possible role of cell contractility in tuning sfo under external stretch, our experimental data are valuable to understand the predominant factor controlling sfo response to exogenous uniaxial stretch, and thus helpful for improving mechanical models.