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مجله زیست شناسی اروپای مرکزی 2014
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  • changes in l-phenylalanine ammonia-lyase activity and isoflavone phytoalexins accumulation in soybean seedlings infected with sclerotinia sclerotiorum

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    • تاریخ ارائه: 1392/07/24
    • تاریخ انتشار در تی پی بین: 1392/07/24
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     soybean [glycine max (l.) merr.] cultivars (meli, alisa, sava and 1511/99) were grown up to v1 phase (first trifoliate and one node above unifoliate) and then inoculated with sclerotinia sclerotiorum(lib.) de bary under controlled conditions. changes in l-phenylalanine ammonia-lyase (pal) activity and isoflavone phytoalexins were recorded 12, 24, 48 and 72 h after the inoculation. results showed an increase in pal activity in all four examined soybean cultivars 48 h after the inoculation, being the highest in alisa (2-fold higher). different contents of total daidzein, genistein, glycitein and coumestrol were detected in all samples. alisa and sava increased their total isoflavone content (33.9% and 6.2% higher than control, respectively) as well as 1511/99, although 48 h after the inoculation its content decreased significantly. meli exhibited the highest rate of coumestrol biosynthesis (72 h after the inoculation) and pal activity (48 h after the inoculation). all investigated cultivars are invariably susceptible to this pathogen. recorded changes could point to possible differences in mechanisms of tolerance among them.

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